Internalization Assay Services of Radiolabeled Compounds
At Rdcthera, we are experts in offering the full spectrum of radiopharmaceutical discovery and development services. We offer comprehensive internalization assay services , which ensures our clients get top-tier support in the development of their drug.
Introduction to Internalization Assays
Internalization means the uptake of substances, including radiopharmaceuticals, by the cells through the cell membrane. An in vitro internalization assay is important in assessing the activity of radiopharmaceuticals. Such an assay would reflect the way a radiopharmaceutical can effectively get into target cells, which was shown to be largely predictive of its eventual therapeutic efficiency and safety.
Fig. 1. Internalization assay reveals resonator uptake by different cell types. (Schubert M, et al., 2017)
Our Internalization Assay Services
We, Rdcthera, offer a full spectra of internalization assays, like receptor-mediated internalization, endocytosis, and intracellular trafficking assays. Our efficient team of scientists uses state-of-the-art instruments and techniques in an effort to provide accurate and reliable data related to the internalization of radiopharmaceuticals for project customers.
With our cell-based assay, you receive the information on the kinetics of internalization and the rate of radiolabelled compound internalization. Our internalization analysis can tell the difference between the radiolabelled compounds associated only with cell surface receptors and the fraction taken up.
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We design and develop assays specific to the properties of your radiopharmaceutical. |
- High-Throughput Screening
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The most modern facilities for rapid, parallel screening of vast numbers of compounds ensure prompt identification of interesting candidates. |
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We can help you with detailed quantitative data regarding the internalization rates and mechanisms of your radiopharmaceuticals. |
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We provide the extent of radiopharmaceuticals taken by target cells under study, hence furnishing very relevant data on cellular absorption and bioavailability. |
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We use the acid wash procedures to distinguish the surface-bound and internalized radiopharmaceuticals and provide information regarding cellular uptake dynamics. |
Our Internalization Analysis Methodologies
We use techniques that apply advanced methodologies to ensure effective, accurate, and reliable internalization analysis.
- Radiolabeling Techniques
- Cellular Assays
- Advanced Imaging
Internalization Assay Validation
Validation is part of our internalization assay process. Every one of the assays is extensively validated to establish its reliability and reproducibility for established radiopharmaceuticals. Therefore, you are assured that the data produced are accurate and actional, thus very important in making a decision in your drug development process.
Our Technological Platform
We leverage cutting-edge technology platforms to support our internalization assays.
- Automated Liquid Handling Systems
- High-Performance Liquid Chromatography
- Flow Cytometry
- Gamma Counters
- Confocal Microscopy
Applications of Internalization Analysis
- Target Validation
- Mechanism of Action Studies
- Optimization of Drug Candidates
- Safety and Efficacy Assessment
To learn more about our internalization assay services and how Rdcthera can support your radiopharmaceutical development efforts, please contact us. Our team is ready to provide the expertise and resources you need to advance your research.
Reference
- Schubert M, Volckaert K, Karl M, et al. (2017). Lasing in Live Mitotic and Non-Phagocytic Cells by Efficient Delivery of Microresonators. Sci Rep. 7:40877.
For research use only. Not intended for any clinical use.
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